Is higher number of different folders affecting website load speed?

I have one question about page load speed. Consider a case when I have different kinds of images on my web page, such as icons, logos, images inside content etc....

I want to know whether having separate folders for each media category may affect the page load speed:

/logos
/icons
/images

Will the webpage load faster if the images of all categories were located in a single folder rather in multiple ones?

Thanks in advance for your advice.