Would it be a performance benefit to disclaim subfolders in a (web development) project folder?

Just as a consideration:

If I would put all my files (images, stylesheets, javascript, icons ...) together with the index.html file direct into the project folder. Using no subfolder to structure my files like imgs, css etc.

Would that be a performance benefit?

I mean: Sure. It would be a big chaos. But it wouldn't be necessary to resolve paths like js/vendors/jquery.js

I'm not sure at all if that resolving path operations are very costly?

Perhaps someone more experienced with performance can say something concerning the idea. Would appreciate it.